AUTHOR=Ferreira Beatriz H. , Ramírez-Prado Jorge H. , Neves Gabriela W. P. , Torrado Egídio , Sampaio Paula , Felipe Maria Sueli S. , Vasconcelos Ana Tereza , Goldman Gustavo H. , Carvalho Agostinho , Cunha Cristina , Lopes-Bezerra Leila M. , Rodrigues Fernando 

TITLE=Ploidy Determination in the Pathogenic Fungus Sporothrix spp.

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00284

DOI=10.3389/fmicb.2019.00284

ISSN=1664-302X

ABSTRACT=<p>The pathogenic clade of the <italic>Sporothrix</italic> genus comprises the etiological agents of sporotrichosis, a worldwide emergent disease. Despite the growing understanding of their successful pathogen traits, there is little information on genome sizes and ploidy within the genus. Therefore, in this work, we evaluated the ploidy of four species of the <italic>Sporothrix</italic> genus, specifically <italic>Sporothrix brasiliensis</italic>, <italic>Sporothrix schenckii</italic>, <italic>Sporothrix globosa</italic>, and <italic>Sporothrix pallida</italic>. Through cell cycle analysis of the yeast-phase cells, we showed that the DNA content of G<sub>0</sub>/G<sub>1</sub> cells was similar to the genome size determined by whole genome sequencing. Moreover, ploidy of <italic>S. schenckii</italic>, <italic>S. brasiliensis</italic>, and <italic>S. pallida</italic> that was determined by allele composition using next-generation sequencing (NGS) data is consistent with monomorphic positions at each allele. These data show that the analyzed strains of <italic>Sporothrix</italic> are haploid, or at least aneuploid, thereby laying the foundation for the development of a molecular toolbox for <italic>Sporothrix</italic> spp.</p>