AUTHOR=Basera Priyanka , Lavania Meeta , Agnihotri Anil , Lal Banwari 

TITLE=Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00282

DOI=10.3389/fmicb.2019.00282

ISSN=1664-302X

ABSTRACT=<p>Indian biodiversity is a hub for medicinal plants. Extensive research has been carried out to select plants with numerous properties which can be used for human welfare. Present research is about <italic>Cymbopogon citratus</italic>, an economically valuable medicinal plant. In this study <italic>Cymbopogon citratus</italic> was elected as a subject plant over the five selected plants (<italic>Azadirachta indica</italic>, <italic>Plumeria obtuse</italic>, <italic>Sapindus mukorossi</italic>, <italic>Capsicum annuum</italic> and <italic>Phyllanthus emblica</italic>) on the basis of antibacterial effect against dominating pathogenic species of gram positive (<italic>Bacillus cereus, Bacillus licheniformis</italic>) and gram negative (<italic>Pseudomonas aeruginosa, Escherichia coli</italic>) bacteria. Further, bioactive agents behind antibacterial potential of <italic>Cymbopogon citratus</italic> was analyzed using analytical method (Phyto-chemical, FTIR, NMR and GC-MS). Due to the broad antimicrobial spectrum, silver nanoparticles have turned into a noteworthy decision for the improvement of new medication. Therefore, this investigation further elaborated in the development of <italic>Cymbopogon citratus</italic> silver nano-particles (CNPs). Antibacterial potential of CNPs examine in a range of C<sub>25</sub>–C<sub>150</sub> (μg/ml) through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) where, C<sub>25</sub> (μg/ml) concentration of CNPs were recorded as the MIC for all bacterial species and C<sub>25</sub> (μg/ml) and C<sub>50</sub> (μg/ml) noted as the MBC for <italic>Pseudomonas aeruginosa, Escherichia coli</italic> and <italic>Bacillus cereus, Bacillus licheniformis</italic>, respectively. In agar disk diffusion assay of CNPs, maximum diameter of zone of inhibition was observed for C<sub>150</sub> (μg/ml) concentration <italic>Bacillus cereus</italic> (20.12 ± 0.42), <italic>Bacillus licheniformis</italic> (22.34 ± 0.4), <italic>Pseudomonas aeruginosa</italic> (35.23 ± 0.46) and <italic>Escherichia coli</italic> (31.87 ± 0.24). Involvement of bioactive component as a reducing and capping agent can be confirmed through FTIR spectrum of CNPs. Moreover XRD, EDXRF and SEM showed crystalline and cuboidal nature of CNPs with <sub>∼</sub>35 nm sizes. Prominently, cytotoxic analysis was conducted to understand the toxic effect of CNPs. This research highlights the potential of CNPs due to the bioactive components present in <italic>Cymbopogon citratus</italic> extract: Polyphenols (phenol; 1584.56 ± 16.32 mg/L, Flavanoids) and mixture of terpenoids (Citral, Myrcene, Farnesol, β-myrcene and β –Pinene)</p>