AUTHOR=Xie Tengfei , Pang Rui , Wu Qingping , Zhang Juemei , Lei Tao , Li Yanping , Wang Juan , Ding Yu , Chen Moutong , Bai Jianlin 

TITLE=Cold Tolerance Regulated by the Pyruvate Metabolism in Vibrio parahaemolyticus

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00178

DOI=10.3389/fmicb.2019.00178

ISSN=1664-302X

ABSTRACT=<p><italic>Vibrio parahaemolyticus</italic> is a common foodborne pathogen found in seafood, and represents a major threat to human health worldwide. Low-temperature storage is an important seafood processing method, but is not sufficient to completely eliminate the bacteria and avoid foodborne illness. To determine the mechanisms behind such cold tolerance, RNA-seq and iTRAQ analyses were first performed to obtain the global transcriptomic and proteomic patterns of frozen squid and clinical <italic>V. parahaemolyticus</italic> isolates under cold conditions. The integrated analysis revealed the modulation of multiple pathways such as the co-occurrence of down-regulated pyruvate metabolism and up-regulated fatty acid biosynthesis, which likely contribute to <italic>V. parahaemolyticus</italic> cold tolerance. Furthermore, we found that increasing concentrations of pyruvate can reduce the fatty acid content to influence <italic>V. parahaemolyticus</italic> growth in cold conditions. Thus, regulation of pyruvate concentration may be an effective method to control this seafood-borne pathogen.</p>