AUTHOR=Savinova Olga S. , Moiseenko Konstantin V. , Vavilova Ekaterina A. , Chulkin Andrey M. , Fedorova Tatiana V. , Tyazhelova Tatiana V. , Vasina Daria V. 

TITLE=Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00152

DOI=10.3389/fmicb.2019.00152

ISSN=1664-302X

ABSTRACT=<p>Laccase is one of the oldest known and intensively studied fungal enzymes capable of oxidizing recalcitrant lignin-resembling phenolic compounds. It is currently well established that fungal genomes almost always contain several non-allelic copies of laccase genes (laccase multigene families); nevertheless, many aspects of laccase multigenicity, for example, their precise biological functions or evolutionary relationships, are mostly unknown. Here, we present a detailed evolutionary analysis of the <italic>sensu stricto</italic> laccase genes (CAZy – AA1_1) from fungi of the Polyporales order. The conducted analysis provides a better understanding of the Polyporales laccase multigenicity and allows for the systemization of the individual features of different laccase isozymes. In addition, we provide a comparison of the biochemical and catalytic properties of the four laccase isozymes from <italic>Trametes hirsuta</italic> and suggest their functional diversification within the multigene family.</p>