AUTHOR=Hermon Louis , Hellal Jennifer , Denonfoux Jérémie , Vuilleumier Stéphane , Imfeld Gwenaël , Urien Charlotte , Ferreira Stéphanie , Joulian Catherine 

TITLE=Functional Genes and Bacterial Communities During Organohalide Respiration of Chloroethenes in Microcosms of Multi-Contaminated Groundwater

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00089

DOI=10.3389/fmicb.2019.00089

ISSN=1664-302X

ABSTRACT=<p>Microcosm experiments with CE-contaminated groundwater from a former industrial site were set-up to evaluate the relationships between biological CE dissipation, dehalogenase genes abundance and bacterial genera diversity. Impact of high concentrations of PCE on organohalide respiration was also evaluated. Complete or partial dechlorination of PCE, TCE, <italic>cis</italic>-DCE and VC was observed independently of the addition of a reducing agent (Na<sub>2</sub>S) or an electron donor (acetate). The addition of either 10 or 100 μM PCE had no effect on organohalide respiration. qPCR analysis of reductive dehalogenases genes (<italic>pceA, tceA, vcrA</italic>, and <italic>bvcA</italic>) indicated that the version of <italic>pceA</italic> gene found in the genus <italic>Dehalococcoides</italic> [hereafter named <italic>pceA</italic>(Dhc)] and <italic>vcrA</italic> gene increased in abundance by one order of magnitude during the first 10 days of incubation. The version of the <italic>pceA</italic> gene found, among others, in the genus <italic>Dehalobacter, Sulfurospirillum, Desulfuromonas</italic>, and <italic>Geobacter</italic> [hereafter named <italic>pceA</italic>(Dhb)] and <italic>bvcA</italic> gene showed very low abundance. The <italic>tceA</italic> gene was not detected throughout the experiment. The proportion of <italic>pceA</italic>(Dhc) or <italic>vcrA</italic> genes relative to the universal 16S ribosomal RNA (16S rRNA) gene increased by up to 6-fold upon completion of <italic>cis-</italic>DCE dissipation. Sequencing of 16S rRNA amplicons indicated that the abundance of Operational Taxonomic Units (OTUs) affiliated to dehalogenating genera <italic>Dehalococcoides, Sulfurospirillum</italic>, and <italic>Geobacter</italic> represented more than 20% sequence abundance in the microcosms. Among organohalide respiration associated genera, only abundance of <italic>Dehalococcoides</italic> spp. increased up to fourfold upon complete dissipation of PCE and <italic>cis</italic>-DCE, suggesting a major implication of <italic>Dehalococcoides</italic> in CEs organohalide respiration. The relative abundance of <italic>pceA</italic> and <italic>vcrA</italic> genes correlated with the occurrence of <italic>Dehalococcoides</italic> and with dissipation extent of PCE, <italic>cis</italic>-DCE and CV. A new type of dehalogenating <italic>Dehalococcoides</italic> sp. phylotype affiliated to the Pinellas group, and suggested to contain both <italic>pceA</italic>(Dhc) and <italic>vcrA</italic> genes, may be involved in organohalide respiration of CEs in groundwater of the study site. Overall, the results demonstrate <italic>in situ</italic> dechlorination potential of CE in the plume, and suggest that taxonomic and functional biomarkers in laboratory microcosms of contaminated groundwater following pollutant exposure can help predict bioremediation potential at contaminated industrial sites.</p>