AUTHOR=Ma Zhongbao , Ye Chao , Deng Weiwei , Xu Mengmeng , Wang Qiong , Liu Gaoqiang , Wang Feng , Liu Liming , Xu Zhenghong , Shi Guiyang , Ding Zhongyang 

TITLE=Reconstruction and Analysis of a Genome-Scale Metabolic Model of Ganoderma lucidum for Improved Extracellular Polysaccharide Production

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.03076

DOI=10.3389/fmicb.2018.03076

ISSN=1664-302X

ABSTRACT=<p>In this study, we reconstructed for the first time a genome-scale metabolic model (GSMM) of <italic>Ganoderma lucidum</italic> strain CGMCC5.26, termed model <italic>i</italic>ZBM1060, containing 1060 genes, 1202 metabolites, and 1404 reactions. Important findings based on model <italic>i</italic>ZBM1060 and its predictions are as follows: (i) The extracellular polysaccharide (EPS) biosynthetic pathway was elucidated completely. (ii) A new fermentation strategy is proposed: addition of phenylalanine increased EPS production by 32.80% in simulations and by 38.00% in experiments. (iii) Eight genes for key enzymes were proposed for EPS overproduction. Model <italic>i</italic>ZBM1060 provides a useful platform for regulating EPS production in terms of system metabolic engineering for <italic>G. lucidum</italic>, as well as a guide for future metabolic pathway construction of other high value-added edible/ medicinal mushroom species.</p>