AUTHOR=Hänel Ingrid , Hotzel Helmut , Tomaso Herbert , Busch Anne 

TITLE=Antimicrobial Susceptibility and Genomic Structure of Arcobacter skirrowii Isolates

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.03067

DOI=10.3389/fmicb.2018.03067

ISSN=1664-302X

ABSTRACT=<p><italic>Campylobacter</italic> spp. are considered the most common bacterial cause of foodborne gastroenteritis in the world. The family <italic>Campylobacteraceae</italic> includes the genus <italic>Arcobacter</italic> with the three species <italic>Arcobacter butzleri</italic>, <italic>Arcobacter cryaerophilus</italic>, and <italic>Arcobacter skirrowii</italic> as emergent enteropathogens and potential zoonotic agents. Here, we characterized genome sequences of <italic>Arcobacter</italic> that were isolated from water poultry on farms in Germany. Isolates were cultured, identified by MALDI-TOF MS and identification was verified with PCR assays. Antibiotic susceptibility testing of isolates was carried out with erythromycin, ciprofloxacin, doxycycline, tetracycline, gentamicin, and streptomycin using the gradient strip method (<italic>E</italic>-test). We also sequenced whole genomes and predicted antibiotic resistance determinants, virulence factors, performed a phylogenetic analysis to determine the genetic relatedness of these isolates and searched for plasmids.</p>