AUTHOR=Silva Higor Oliveira , Lima Joyce Aparecida Santos , Aguilar Carlos Eduardo Gamero , Rossi Gabriel Augusto Marques , Mathias Luis Antonio , Vidal Ana Maria Centola 

TITLE=Efficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact With Milk

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02934

DOI=10.3389/fmicb.2018.02934

ISSN=1664-302X

ABSTRACT=<p>The species of the <italic>Bacillus cereus</italic> group have the ability to adhere to and form biofilms on solid surfaces, including stainless steel, a material widely used in food industries. Biofilms allow for recontamination during food processing, and the “clean-in-place” (CIP) system is largely used by industries to control them. This study thus proposes to evaluate the efficacy of peracetic acid and sodium hypochlorite against biofilms induced on stainless-steel surfaces. The SAMN07414939 isolate (BioProject <ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="PRJNA390851" xmlns:xlink="http://www.w3.org/1999/xlink">PRJNA390851</ext-link>), a recognized biofilm producer, was selected for biofilm induction on AISI 304 stainless steel. Biofilm induction was performed and classified into three categories: TCP (Tindalized, Contaminated, and Pasteurized milk), TCS (Tindalized milk Contaminated with Spores), and TCV (Tindalized milk Contaminated with Vegetative cells). Subsequently, the coupons were sanitized simulating a CIP procedure, on a pilot scale, using alkaline and acid solutions followed by disinfectants (peracetic acid and sodium hypochlorite). Microorganism adhesion on the surfaces reached 6.3 × 10<sup>5</sup> to 3.1 × 10<sup>7</sup> CFU/cm<sup>-2</sup>. Results did not show significant differences (<italic>p</italic> &gt; 0.05) for surface adhesion between the three tested categories (TCP, TCS, and TCV) or (<italic>p</italic> &gt; 0.05) between the two disinfectants (peracetic acid and sodium hypochlorite). Microbial populations adhered to the stainless-steel coupons are equally reduced after treatment with peracetic acid and sodium hypochlorite, with no differences in the control of <italic>B. cereus</italic><italic>s.s.</italic> biofilms on AISI 304 stainless-steel surfaces.</p>