AUTHOR=Lin Aiyang , Zeng Chuanwen , Wang Qian , Zhang Wenqing , Li Mengyao , Hanna Michelle , Xiao Wei 

TITLE=Utilization of a Strongly Inducible DDI2 Promoter to Control Gene Expression in Saccharomyces cerevisiae

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02736

DOI=10.3389/fmicb.2018.02736

ISSN=1664-302X

ABSTRACT=<p>Regulating target gene expression is a common method in yeast research. In <italic>Saccharomyces cerevisiae</italic>, there are several widely used regulated expression systems, such as the <italic>GAL</italic> and Tet-off systems. However, all current expression systems possess some intrinsic deficiencies. We have previously reported that the <italic>DDI2</italic> gene can be induced to very high levels upon cyanamide or methyl methanesulfonate treatment. Here we report the construction of gene expression systems based on the <italic>DDI2</italic> promoter in both single- and multi-copy plasmids. Using <italic>GFP</italic> as a reporter gene, it was demonstrated that the target gene expression could be increased by up to 2,000-fold at the transcriptional level by utilizing the above systems. In addition, a <italic>DDI2</italic>-based construct was created for promoter shuffling in the budding yeast genome to control endogenous gene expression. Overall, this study offers a set of convenient and highly efficient experimental tools to control target gene expression in budding yeast.</p>