AUTHOR=Liu Lei , Wu Ruiyun , Zhang Jinlan , Li Pinglan 

TITLE=Overexpression of luxS Promotes Stress Resistance and Biofilm Formation of Lactobacillus paraplantarum L-ZS9 by Regulating the Expression of Multiple Genes

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02628

DOI=10.3389/fmicb.2018.02628

ISSN=1664-302X

ABSTRACT=<p>Probiotics have evoked great interest in the past years for their beneficial effects. The aim of this study was to investigate whether <italic>luxS</italic> overexpression promotes the stress resistance of <italic>Lactobacillus paraplantarum</italic> L-ZS9. Here we show that overexpression of <italic>luxS</italic> gene increased the production of autoinducer-2 (AI-2, quorum sensing signal molecule) by <italic>L. paraplantarum</italic> L-ZS9. At the same time, overexpression of <italic>luxS</italic> promoted heat-, bile salt-resistance and biofilm formation of the strain. RNAseq results indicated that multiple genes encoding transporters, membrane proteins, and transcriptional regulator were regulated by <italic>luxS</italic>. These results reveal a new role for LuxS in promoting stress resistance and biofilm formation of probiotic starter.</p>