AUTHOR=Fang Wensheng , Yan Dongdong , Wang Xianli , Huang Bin , Wang Xiaoning , Liu Jie , Liu Xiaoman , Li Yuan , Ouyang Canbin , Wang Qiuxia , Cao Aocheng 

TITLE=Responses of Nitrogen-Cycling Microorganisms to Dazomet Fumigation

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02529

DOI=10.3389/fmicb.2018.02529

ISSN=1664-302X

ABSTRACT=<p>The influence of soil fumigation on microorganisms involved in transforming nitrogen remains little understood, despite the use of fumigants for many decades to control soil-borne pathogens and plant-parasitic nematodes. We used real-time PCR (quantitative polymerase chain reaction) and 16S rRNA gene amplicon sequencing techniques to monitor changes in the diversity and community structure of microorganisms associated with nitrogen transfer after the soil was fumigated with dazomet (DZ). We also examined nitrous oxide (N<sub>2</sub>O) emissions from these microorganisms present in fumigated fluvo-aquic soil and lateritic red soil. Fumigation with DZ significantly reduced the abundance of 16S rRNA and nitrogen cycling functional genes (<italic>nifH</italic>, AOA <italic>amoA</italic>, AOB <italic>amoA, nxrB, narG, napA, nirK, nirS, cnorB, qnorB</italic>, and <italic>nosZ</italic>). At the same time, N<sub>2</sub>O production rates increased between 9.9 and 30 times after fumigation. N<sub>2</sub>O emissions were significantly correlated with <inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mi mathvariant="normal" mathcolor="black">NH</mml:mi><mml:mi mathvariant="normal" mathcolor="black">4</mml:mi><mml:mo mathvariant="normal" mathcolor="black">+</mml:mo></mml:msubsup></mml:math></inline-formula>, dissolved amino acids and microbial biomass nitrogen, but uncorrelated with functional gene abundance. Diversity indices showed that DZ temporarily stimulated bacterial diversity as well as caused a significant change in bacterial community composition. For example, DZ significantly decreased populations of N<sub>2</sub>-fixing bacteria <italic>Mesorhizobium</italic> and <italic>Paenibacillus</italic>, nitrifiers <italic>Nitrosomonas</italic>, and the denitrifiers <italic>Bacillus, Pseudomonas</italic>, and <italic>Paracoccus.</italic> The soil microbial community had the ability to recover to similar population levels recorded in unfumigated soils when the inhibitory effects of DZ fumigation were no longer evident. The microbial recovery rate, however, depended on the physicochemical properties of the soil. These results provided useful information for environmental safety assessments of DZ in China, for improving our understanding of the N-cycling pathways in fumigated soils, and for determining the potential responses of different N-cycling groups after fumigation.</p>