AUTHOR=Thi Bach Nguyen Hue , Romero A. David , Amman Fabian , Sorger-Domenigg Theresa , Tata Muralidhar , Sonnleitner Elisabeth , Bläsi Udo 

TITLE=Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02488

DOI=10.3389/fmicb.2018.02488

ISSN=1664-302X

ABSTRACT=<p><italic>Pseudomonas aeruginosa</italic> (Pae) is an opportunistic human pathogen, able to resist host defense mechanisms and antibiotic treatment. In Pae, the master regulator of stress responses RpoS (σ<sup>S</sup>) is involved in the regulation of quorum sensing and several virulence genes. Here, we report that the sRNA ReaL translationally silences <italic>rpoS</italic> mRNA, which results in a decrease of the RpoS levels. Our studies indicated that ReaL base-pairs with the Shine-Dalgarno region of <italic>rpoS</italic> mRNA. These studies are underlined by a highly similar transcription profile of a <italic>rpoS</italic> deletion mutant and a <italic>reaL</italic> over-expressing strain.</p>