AUTHOR=Dai Daofeng , Pu Tianning , Liang Jingdan , Wang Zhijun , Tang Aifa 

TITLE=Regulation of dndB Gene Expression in Streptomyces lividans

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02387

DOI=10.3389/fmicb.2018.02387

ISSN=1664-302X

ABSTRACT=<p>DNA sulfur modification is a unique modification occurring in the sugar-phosphate backbone of DNA, with a nonbridging oxygen atom substituted with sulfur in a sequence-specific and <italic>R</italic>p stereo-specific manner. Bioinformatics, RNA-seq, and <italic>in vitro</italic> transcriptional analyses have shown that DNA sulfur modification may be involved in epigenetic regulation. However, the <italic>in vivo</italic> evidence supporting this assertion is not convincing. Here, we aimed to characterize two sulfur-modified sites near the <italic>dndB</italic> promoter region in <italic>Streptomyces lividans</italic>. Single mutation of either site had no effect on <italic>dndB</italic> transcription, whereas double mutation of both sites significantly elevated <italic>dndB</italic> expression. These findings suggested that DNA sulfur modification affected gene expression, and the role of DNA sulfur modification in epigenetic regulation depended on the number of sulfur-modified sites. We also identified an inverted repeat, the R repeat sequence, and showed that this sequence participated in the positive regulation of <italic>dndB</italic> gene expression.</p>