AUTHOR=Luz Nivea F. , Khouri Ricardo , Van Weyenbergh Johan , Zanette Dalila L. , Fiuza Paloma P. , Noronha Almerio , Barral Aldina , Boaventura Viviane S. , Prates Deboraci B. , Chan Francis Ka-Ming , Andrade Bruno B. , Borges Valeria M. TITLE=Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression JOURNAL=Frontiers in Microbiology VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02283 DOI=10.3389/fmicb.2018.02283 ISSN=1664-302X ABSTRACT=

Leishmania braziliensis infection causes skin ulcers, typically found in localized cutaneous leishmaniasis (LCL). This tissue pathology associates with different modalities of cell necrosis, which are subverted by the parasite as a survival strategy. Herein we examined the participation of necroptosis, a specific form of programmed necrosis, in LCL lesions and found reduced RIPK3 and PGAM5 gene expression compared to normal skin. Assays using infected macrophages demonstrated that the parasite deactivates both RIPK3 and MLKL expression and that these molecules are important to control the intracellular L. braziliensis replication. Thus, LCL-related necroptosis may be targeted to control infection and disease immunopathology.