AUTHOR=Semedo Miguel , Song Bongkeun , Sparrer Tavis , Phillips Rebecca L. TITLE=Antibiotic Effects on Microbial Communities Responsible for Denitrification and N2O Production in Grassland Soils JOURNAL=Frontiers in Microbiology VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02121 DOI=10.3389/fmicb.2018.02121 ISSN=1664-302X ABSTRACT=
Antibiotics in soils may affect the structure and function of microbial communities. In this study, we investigated the acute effects of tetracycline on soil microbial community composition and production of nitrous oxide (N2O) and dinitrogen (N2) as the end-products of denitrification. Grassland soils were pre-incubated with and without tetracycline for 1-week prior to measurements of N2O and N2 production in soil slurries along with the analysis of prokaryotic and fungal communities by quantitative polymerase chain reaction (qPCR) and next-generation sequencing. Abundance and taxonomic composition of bacteria carrying two genotypes of N2O reductase genes (