AUTHOR=Potter Robert F. , D'Souza Alaric W. , Wallace Meghan A. , Shupe Angela , Patel Sanket , Gul Danish , Kwon Jennie H. , Beatty Wandy , Andleeb Saadia , Burnham Carey-Ann D. , Dantas Gautam 

TITLE=Superficieibacter electus gen. nov., sp. nov., an Extended-Spectrum β-Lactamase Possessing Member of the Enterobacteriaceae Family, Isolated From Intensive Care Unit Surfaces

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01629

DOI=10.3389/fmicb.2018.01629

ISSN=1664-302X

ABSTRACT=<p>Two Gram-negative bacilli strains, designated BP-1(T) and BP-2, were recovered from two different Intensive Care Unit surfaces during a longitudinal survey in Pakistan. Both strains were unidentified using the bioMerieux VITEK MS IVD v2.3.3 and Bruker BioTyper MALDI-TOF mass spectrometry platforms. To more precisely determine the taxonomic identity of BP-1(T) and BP-2, we employed a biochemical and phylogenomic approach. The 16S rRNA gene sequence of strain BP-1(T) had the highest identity to <italic>Citrobacter farmeri</italic> CDC 2991-81(T) (98.63%) <italic>Citrobacter amalonaticus</italic> CECT 863(T) (98.56%), <italic>Citrobacter sedlakii</italic> NBRC 105722(T) (97.74%) and <italic>Citrobacter rodentium</italic> NBRC 105723(T) (97.74%). The biochemical utilization scheme of BP-1(T) using the Analytic Profile Index for Enterobacteriaceae (API20E) indicated its enzymatic functions are unique within the Enterobacteriaceae but most closely resemble <italic>Kluyvera</italic> spp., <italic>Enterobacter cloacae</italic> and <italic>Citrobacter koseri/farmeri</italic>. Phylogenomic analysis of the shared genes between BP-1(T), BP-2 and type strains from <italic>Kluyvera, Citrobacter, Escherichia, Salmonella, Kosakonia, Siccibacter</italic> and <italic>Shigella</italic> indicate that BP-1(T) and BP-2 isolates form a distinct branch from these genera. Average Nucleotide Identity analysis indicates that BP-1(T) and BP-2 are the same species. The biochemical and phylogenomic analysis indicate strains BP-1(T) and BP-2 represent a novel species from a new genus within the Enterobacteriaceae family, for which the name <italic>Superficieibacter electus</italic> gen. nov., sp. nov., is proposed. The type strain is BP-1(T) (= ATCC BAA-2937, = NBRC 113412).</p>