AUTHOR=Lazarevic Vladimir , Gaïa Nadia , Girard Myriam , Leo Stefano , Cherkaoui Abdessalam , Renzi Gesuele , Emonet Stéphane , Jamme Sharon , Ruppé Etienne , Vijgen Sandrine , Rubbia-Brandt Laura , Toso Christian , Schrenzel Jacques TITLE=When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing JOURNAL=Frontiers in Microbiology VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01566 DOI=10.3389/fmicb.2018.01566 ISSN=1664-302X ABSTRACT=

Here, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun sequencing may be used together with other tests to strengthen the diagnosis of hepatic brucelloma.