AUTHOR=Nascimento Altina Lacerda , Souza Adijailton Jose , Andrade Pedro Avelino Maia , Andreote Fernando Dini , Coscione Aline Renée , Oliveira Fernando Carvalho , Regitano Jussara Borges 

TITLE=Sewage Sludge Microbial Structures and Relations to Their Sources, Treatments, and Chemical Attributes

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01462

DOI=10.3389/fmicb.2018.01462

ISSN=1664-302X

ABSTRACT=<p>Sewage sludges generation and their disposal have become one of the greatest challenges of the 21st century. They have great microbial diversity that may impact wastewater treatment plant (WWTP) efficiency and soil quality whether used as fertilizers. Therefore, this research aimed to characterize microbial community diversity and structure of 19 sewage sludges from São Paulo, Brazil, as well as to draw their relations to sludge sources [domestic and mixed (domestic+industrial)], biological treatments (redox conditions and liming), and chemical attributes, using molecular biology as a tool. All sludges revealed high bacterial diversity, but their sources and redox operating conditions as well as liming did not consistently affect bacterial community structures. <italic>Proteobacteria</italic> was the dominant phylum followed by <italic>Bacteroidetes</italic> and <italic>Firmicutes</italic>; whereas <italic>Clostridium</italic> was the dominant genus followed by <italic>Treponema</italic>, <italic>Propionibacterium</italic>, <italic>Syntrophus</italic>, and <italic>Desulfobulbus</italic>. The sludge samples could be clustered into six groups (C1 to C6) according their microbial structure similarities. Very high pH (≥11.9) was the main sludge attribute segregating C6, that presented very distinct microbial structure from the others. Its most dominant genera were <italic>Propionibacterium</italic> &gt; &gt; <italic>Comamonas</italic> &gt; <italic>Brevundimonas</italic> &gt; <italic>Methylobacterium</italic> ∼<italic>Stenotrophomonas</italic> ∼<italic>Cloacibacterium</italic>. The other clusters’ dominant genera were <italic>Clostridium</italic> &gt; &gt; <italic>Treponema</italic> &gt; <italic>Desulfobulbus</italic> ∼<italic>Syntrophus.</italic> Moreover, high Fe and S were important modulators of microbial structure in certain sludges undertaking anaerobic treatment and having relatively low N-Kj, B, and P contents (C5). However, high N-Kj, B, P, and low Fe and Al contents were typical of domestic, unlimed, and aerobically treated sludges (C1). In general, heavy metals had little impact on microbial community structure of the sludges. However, our sludges shared a common core of 77 bacteria, mostly <italic>Clostridium</italic>, <italic>Treponema</italic>, <italic>Syntrophus</italic>, and <italic>Comamonas</italic>. They should dictate microbial functioning within WWTPs, except by SS12 and SS13.</p>