AUTHOR=Raymond Eder María L. , Conti Francisco , Rosa Alberto L. 

TITLE=Differences Between Indigenous Yeast Populations in Spontaneously Fermenting Musts From V. vinifera L. and V. labrusca L. Grapes Harvested in the Same Geographic Location

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01320

DOI=10.3389/fmicb.2018.01320

ISSN=1664-302X

ABSTRACT=<p>Yeast communities associated with <italic>Vitis vinifera</italic> L. ecosystems have been widely characterized. Less is known, however, about yeast communities present in grapes and fermenting musts from <italic>Vitis</italic> non-<italic>vinifera</italic> ecosystems. Moreover, there are no comparative studies concerning yeast communities in grapes from <italic>V. vinifera</italic> L. and non-<italic>vinifera Vitis</italic> species in vineyards from a shared <italic>terroir</italic>. In this work, we have used a culture-dependent strategy, phenotypic analyses, and molecular genotyping, to study the most representative yeast species present in spontaneously fermenting musts of grapes harvested from neighboring <italic>V. vinifera</italic> L. (cv. Malbec) and <italic>V. labrusca</italic> L. (cv. Isabella) vineyards. Phenotypic analyses of H<sub>2</sub>S production, ethanol tolerance and carbon utilization, on randomly selected strains of each <italic>Hanseniaspora uvarum, Starmerella bacillaris</italic> and <italic>Saccharomyces cerevisiae</italic> strains, as well as microsatellite genotyping of <italic>S. cerevisiae</italic> isolates from each the Malbec and Isabella grape musts, suggest that <italic>V. vinifera</italic> L. and <italic>V. labrusca</italic> L. ecosystems could harbor different yeast strain populations. Thus, microbial communities in exotic <italic>Vitis</italic> species may offer opportunities to look for unique yeast strains that could not be present in conventional <italic>V. vinifera</italic> L. ecosystems.</p>