AUTHOR=Sarwar Arslan , Latif Zakia , Zhang Songya , Zhu Jing , Zechel David L. , Bechthold Andreas 

TITLE=Biological Control of Potato Common Scab With Rare Isatropolone C Compound Produced by Plant Growth Promoting Streptomyces A1RT

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01126

DOI=10.3389/fmicb.2018.01126

ISSN=1664-302X

ABSTRACT=<p>Potato is prone to many drastic diseases like potato common scab (CS). As no highly effective methods exist for managing CS, this study explored the possibility of using biological control. Ten bacterial strains were isolated from CS-infected potato tubers from four different locations of Punjab, Pakistan, and identified based on biochemical and molecular analysis. Analysis of 16s rDNA sequences amplified by PCR revealed the isolated bacterial strains to be <italic>Streptomyces scabies, S. turgidiscabies</italic> and <italic>S. stelliscabiei</italic>. Pathogenic islands were also confirmed among the isolates after identification of <italic>txtAB, nec1</italic>, and <italic>tomA</italic> genes with PCR amplification. One strain isolated from soil was antagonistic to the pathogenic <italic>Streptomyces</italic> spp., and determined to be <italic>Streptomyces</italic> A1RT on the basis of 16s rRNA sequencing. A methanolic extract of <italic>Streptomyces</italic> A1RT contained Isatropolone C, which was purified and structurally determined by <sup>1</sup>H- and <sup>13</sup>C-NMR, <sup>1</sup>H/<sup>1</sup>H-COSY, HMQC, and HMBC techniques. <italic>Streptomyces</italic> A1RT also produced the plant growth hormone indole-3-acetic acid (IAA) with a titer of 26 μg ml<sup>-1</sup> as confirmed by spectrophotometry and HPLC. In a greenhouse assay, disease severity index was established from 0 to 500. Average disease severity indexes were recorded as 63, 130.5, and 78 for <italic>Streptomyces scabies, S. turgidiscabies</italic> and <italic>S. stelliscabiei</italic>, respectively. When <italic>Streptomyces</italic> A1RT was applied in soil that contained one of these pathogenic isolates, the average disease severity indexes were significantly (<italic>P</italic> &lt; 0.05) reduced to 11.1, 5.6 and 8.4, respectively. A significant increase in tuber weight and shoot development was also observed with the tubers treated with <italic>Streptomyces</italic> A1RT. The use of the plant growth-promoting <italic>Streptomyces</italic> A1RT against potato CS thus provides an alternative strategy to control the disease without affecting environmental, plants, animals and human health.</p>