AUTHOR=Kim Hey-Min , Yoon Chang-Kyu , Ham Hyeong-In , Seok Yeong-Jae , Park Young-Ha 

TITLE=Stimulation of Vibrio vulnificus Pyruvate Kinase in the Presence of Glucose to Cope With H2O2 Stress Generated by Its Competitors

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01112

DOI=10.3389/fmicb.2018.01112

ISSN=1664-302X

ABSTRACT=<p>The bacterial phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) regulates a variety of cellular processes in addition to catalyzing the coupled transport and phosphorylation of carbohydrates. We recently reported that, in the presence of glucose, HPr of the PTS is dephosphorylated and interacts with pyruvate kinase A (PykA) catalyzing the conversion of PEP to pyruvate in <italic>Vibrio vulnificus</italic>. Here, we show that this interaction enables <italic>V. vulnificus</italic> to survive H<sub>2</sub>O<sub>2</sub> stress by increasing pyruvate production. A <italic>pykA</italic> deletion mutant was more susceptible to H<sub>2</sub>O<sub>2</sub> stress than wild-type <italic>V. vulnificus</italic> without any decrease in the expression level of catalase, and this sensitivity was rescued by the addition of pyruvate. The H<sub>2</sub>O<sub>2</sub> sensitivity difference between wild-type and <italic>pykA</italic> mutant strains becomes more apparent in the presence of glucose. Fungi isolated from the natural habitat of <italic>V. vulnificus</italic> retarded the growth of the <italic>pykA</italic> mutant more severely than the wild-type strain in the presence of glucose by glucose oxidase-dependent generation of H<sub>2</sub>O<sub>2</sub>. These data suggest that <italic>V. vulnificus</italic> has evolved to resist the killing action of its fungal competitors by increasing pyruvate production in the presence of glucose.</p>