AUTHOR=Mulot Michaël , Monsion Baptiste , Boissinot Sylvaine , Rastegar Maryam , Meyer Sophie , Bochet Nicole , Brault Véronique 

TITLE=Transmission of Turnip yellows virus by Myzus persicae Is Reduced by Feeding Aphids on Double-Stranded RNA Targeting the Ephrin Receptor Protein

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.00457

DOI=10.3389/fmicb.2018.00457

ISSN=1664-302X

ABSTRACT=<p>Aphid-transmitted plant viruses are a threat for major crops causing massive economic loss worldwide. Members in the <italic>Luteoviridae</italic> family are transmitted by aphids in a circulative and non-replicative mode. Virions are acquired by aphids when ingesting sap from infected plants and are transported through the gut and the accessory salivary gland (ASG) cells by a transcytosis mechanism relying on virus-specific receptors largely unknown. Once released into the salivary canal, virions are inoculated to plants, together with saliva, during a subsequent feeding. In this paper, we bring <italic>in vivo</italic> evidence that the membrane-bound Ephrin receptor (Eph) is a novel aphid protein involved in the transmission of the <italic>Turnip yellows virus</italic> (TuYV, <italic>Polerovirus</italic> genus, <italic>Luteoviridae</italic> family) by <italic>Myzus persicae</italic>. The minor capsid protein of TuYV, essential for aphid transmission, was able to bind the external domain of Eph in yeast. Feeding <italic>M. persicae</italic> on <italic>in planta</italic>- or <italic>in vitro</italic>-synthesized dsRNA targeting <italic>Eph</italic>-mRNA (dsRNA<sub>Eph</sub>) did not affect aphid feeding behavior but reduced accumulation of TuYV genomes in the aphid's body. Consequently, TuYV transmission efficiency by the dsRNA<sub>Eph</sub>-treated aphids was reproducibly inhibited and we brought evidence that Eph is likely involved in intestinal uptake of the virion. The inhibition of virus uptake after dsRNA<sub>Eph</sub> acquisition was also observed for two other poleroviruses transmitted by <italic>M. persicae</italic>, suggesting a broader role of Eph in polerovirus transmission. Finally, dsRNA<sub>Eph</sub> acquisition by aphids did not affect nymph production. These results pave the way toward an ecologically safe alternative of insecticide treatments that are used to lower aphid populations and reduce polerovirus damages.</p>