AUTHOR=Botas Alma , Pérez-Redondo Rosario , Rodríguez-García Antonio , Álvarez-Álvarez Rubén , Yagüe Paula , Manteca Angel , Liras Paloma 

TITLE=ArgR of Streptomyces coelicolor Is a Pleiotropic Transcriptional Regulator: Effect on the Transcriptome, Antibiotic Production, and Differentiation in Liquid Cultures

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.00361

DOI=10.3389/fmicb.2018.00361

ISSN=1664-302X

ABSTRACT=<p>ArgR is a well-characterized transcriptional repressor controlling the expression of arginine and pyrimidine biosynthetic genes in bacteria. In this work, the biological role of <italic>Streptomyces coelicolor</italic> ArgR was analyzed by comparing the transcriptomes of <italic>S. coelicolor</italic> Δ<italic>argR</italic> and its parental strain, <italic>S. coelicolor</italic> M145, at five different times over a 66-h period. The effect of <italic>S. coelicolor</italic> ArgR was more widespread than that of the orthologous protein of <italic>Escherichia coli</italic>, affecting the expression of 1544 genes along the microarray time series. This <italic>S. coelicolor</italic> regulator repressed the expression of arginine and pyrimidine biosynthetic genes, but it also modulated the expression of genes not previously described to be regulated by ArgR: genes involved in nitrogen metabolism and nitrate utilization; the <italic>act, red</italic>, and <italic>cpk</italic> genes for antibiotic production; genes for the synthesis of the osmotic stress protector ectoine; genes related to hydrophobic cover formation and sporulation (chaplins, rodlins, <italic>ramR</italic>, and <italic>whi</italic> genes); all the <italic>cwg</italic> genes encoding proteins for glycan cell wall biosynthesis; and genes involved in gas vesicle formation. Many of these genes contain ARG boxes for ArgR binding. ArgR binding to seven new ARG boxes, located upstream or near the <italic>ectA-ectB, afsS, afsR, glnR</italic>, and <italic>redH</italic> genes, was tested by DNA band-shift assays. These data and those of previously assayed fragments permitted the construction of an improved model of the ArgR binding site. Interestingly, the overexpression of sporulation genes observed in the Δ<italic>argR</italic> mutant in our culture conditions correlated with a sporulation-like process, an uncommon phenotype.</p>