AUTHOR=Balay Danielle R. , Gänzle Michael G. , McMullen Lynn M. 

TITLE=The Effect of Carbohydrates and Bacteriocins on the Growth Kinetics and Resistance of Listeria monocytogenes

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.00347

DOI=10.3389/fmicb.2018.00347

ISSN=1664-302X

ABSTRACT=<p>The aim of this study was to determine if different carbohydrates influence the growth of <italic>Listeria monocytogenes</italic> in the presence of carnocyclin A or leucocin A. <italic>Carnobacterium maltaromaticum</italic> ATCC PTA-5313 and <italic>Leuconostoc gelidum</italic> UAL187 were used to produce carnocyclin A and leucocin A, respectively. Growth curves were modeled for five strains of <italic>L. monocytogenes</italic> grown in basal medium supplemented with glucose, sucrose, fructose, mannose, or cellobiose, in the presence of carnocyclin A or leucocin A. The growth of <italic>L. monocytogenes</italic> to leucocin A or carnocyclin A was influenced by carbohydrate and/or strain. Carnocyclin A inhibited the growth of <italic>L. monocytogenes</italic> more than leucocin A. Growth in media containing glucose, mannose, and fructose increased the sensitivity of some strains of <italic>L. monocytogenes</italic> to bacteriocins, while growth in cellobiose and sucrose increased the resistance of <italic>L. monocytogenes</italic> to bacteriocins, as evidenced by a shorter lag phase. Strains of <italic>L. monocytogenes</italic> developed resistance to both leucocin A and carnocyclin A, but the time to develop resistance was longer when strains are treated with carnocyclin A. Carbohydrate influences the development of resistance of <italic>L. monocytogenes</italic> to the bacteriocins, but the ability of strains to develop resistance to leucocin A or carnocyclin A differs. Results of this study indicate that carbohydrates influence the ability of <italic>L. monocytogenes</italic> to grow in the presence of bacteriocins.</p>