AUTHOR=Hao Mengyuan , Cui Yanhua , Qu Xiaojun 

TITLE=Analysis of CRISPR-Cas System in Streptococcus thermophilus and Its Application

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.00257

DOI=10.3389/fmicb.2018.00257

ISSN=1664-302X

ABSTRACT=<p>CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR associated proteins) loci, which provide a specific immunity against exogenous elements, are hypervariable among distinct prokaryotes. Based on previous researches, this review focuses on concluding systematical genome editing protocols in <italic>Streptococcus thermophilus.</italic> Firstly, its protocols and optimized conditions in gene editing are introduced. What’s more, classification and diversity analyses of <italic>S. thermophilus</italic> CRISPR-Cas benefit the further understanding of evolution relationship among <italic>Streptococcus.</italic> Ability of its foreign segment integration and spacer source analyses also indicate a new direction of phage resistance. Above all, all of these point out its potential to be regarded as another model system other than type II CRISPR-Cas in <italic>Streptococcus pyogenes</italic>.</p>