AUTHOR=Wang Jianfeng , Li Hongen , Pan Juan , Dong Jing , Zhou Xuan , Niu Xiaodi , Deng Xuming 

TITLE=Oligopeptide Targeting Sortase A as Potential Anti-infective Therapy for Staphylococcus aureus

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.00245

DOI=10.3389/fmicb.2018.00245

ISSN=1664-302X

ABSTRACT=<p>Sortase A (SrtA)-catalyzed anchorage of surface proteins in most Gram-positive bacteria is indispensable for their virulence, suggesting that this transpeptidase is a promising target for antivirulence therapy. Here, an oligopeptide, LPRDA, was identified as an effective inhibitor of SrtA via virtual screening based on the LPXTG substrate sequence, and it was found to inhibit SrtA activity <italic>in vitro</italic> and <italic>in vivo</italic> (IC<sub>50</sub> = 10.61 μM) by competitively occupying the active site of SrtA. Further, the oligopeptide treatment had no anti-<italic>Staphylococcus aureus</italic> activity, but it provided protection against <italic>S. aureus</italic>-induced mastitis in a mouse model. These findings indicate that the oligopeptide could be used as an effective anti-infective agent for the treatment of infection caused by <italic>S. aureus</italic> or other Gram-positive bacteria via the targeting of SrtA.</p>