AUTHOR=Harris Howard W. , Sánchez-Andrea Irene , McLean Jeffrey S. , Salas Everett C. , Tran William , El-Naggar Mohamed Y. , Nealson Kenneth H. 

TITLE=Redox Sensing within the Genus Shewanella

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.02568

DOI=10.3389/fmicb.2017.02568

ISSN=1664-302X

ABSTRACT=<p>A novel bacterial behavior called congregation was recently described in <italic>Shewanella oneidensis</italic> MR-1 as the accumulation of cells around insoluble electron acceptors (IEA). It is the result of a series of “run-and-reversal” events enabled by modulation of swimming speed and direction. The model proposed that the swimming cells constantly sense their surroundings with specialized outer membrane cytochromes capable of extracellular electron transport (EET). Up to this point, neither the congregation nor attachment behavior have been studied in any other strains. In this study, the wild type of <italic>S. oneidensis</italic> MR-1 and several deletion mutants as well as eight other <italic>Shewanella</italic> strains (<italic>Shewanella putrefaciens</italic> CN32, <italic>S</italic>. sp. ANA-3, <italic>S</italic>. sp. W3-18-1, <italic>Shewanella amazonensis</italic> SB2B, <italic>Shewanella loihica</italic> PV-4, <italic>Shewanella denitrificans</italic> OS217, <italic>Shewanella baltica</italic> OS155, and <italic>Shewanella frigidimarina</italic> NCIMB400) were screened for the ability to congregate. To monitor congregation and attachment, specialized cell-tracking techniques, as well as a novel cell accumulation after photo-bleaching (CAAP) confocal microscopy technique were utilized in this study. We found a strong correlation between the ability of strain MR-1 to accumulate on mineral surface and the presence of key EET genes such as <italic>mtrBC/omcA</italic> (SO_1778, SO_1776, and SO_1779) and gene coding for methyl-accepting protein (MCPs) with <italic>Ca</italic><sup>+</sup> channel <italic>che</italic>motaxis receptor (Cache) domain (SO_2240). These EET and taxis genes were previously identified as essential for characteristic run and reversal swimming around IEA surfaces. CN32, ANA-3, and PV-4 congregated around both Fe(OH)<sub>3</sub> and MnO<sub>2</sub>. Two other <italic>Shewanella</italic> spp. showed preferences for one oxide over the other: preferences that correlated with the metal content of the environments from which the strains were isolated: e.g., W3-18-1, which was isolated from an iron-rich habitat congregated and attached preferentially to Fe(OH)<sub>3</sub>, while SB2B, which was isolated from a MnO<sub>2</sub>-rich environment, preferred MnO<sub>2</sub>.</p>