AUTHOR=Shi Shuobo , Zhao Huimin 

TITLE=Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.02185

DOI=10.3389/fmicb.2017.02185

ISSN=1664-302X

ABSTRACT=<p>Oleaginous yeasts have been increasingly explored for production of chemicals and fuels via metabolic engineering. Particularly, there is a growing interest in using oleaginous yeasts for the synthesis of lipid-related products due to their high lipogenesis capability, robustness, and ability to utilize a variety of substrates. Most of the metabolic engineering studies in oleaginous yeasts focused on <italic>Yarrowia</italic> that already has plenty of genetic engineering tools. However, recent advances in systems biology and synthetic biology have provided new strategies and tools to engineer those oleaginous yeasts that have naturally high lipid accumulation but lack genetic tools, such as <italic>Rhodosporidium</italic>, <italic>Trichosporon</italic>, and <italic>Lipomyces</italic>. This review highlights recent accomplishments in metabolic engineering of oleaginous yeasts and recent advances in the development of genetic engineering tools in oleaginous yeasts within the last 3 years.</p>