AUTHOR=Su Lanxi , Shen Zongzhuan , Ruan Yunze , Tao Chengyuan , Chao Yifan , Li Rong , Shen Qirong 

TITLE=Isolation of Antagonistic Endophytes from Banana Roots against Meloidogyne javanica and Their Effects on Soil Nematode Community

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.02070

DOI=10.3389/fmicb.2017.02070

ISSN=1664-302X

ABSTRACT=<p>Banana production is seriously hindered by <italic>Meloidogyne</italic> spp. all over the world. Endophytes are ideal candidates compared to pesticides as an environmentally benign agent. In the present study, endophytes isolated from banana roots infected by <italic>Meloidogyne</italic> spp. with different disease levels were tested <italic>in vitro</italic>, and in sterile and nature banana monoculture soils against <italic>Meloidogyne javanica</italic>. The proportion of antagonistic endophytes were higher in the roots of middle and high disease levels. Among those, bacteria were dominant, and <italic>Pseudomonas</italic> spp., <italic>Bacillus</italic> spp. and <italic>Streptomyces</italic> spp. showed more abundant populations. One strain, named as SA, with definite root inner-colonization ability was isolated and identified as <italic>Streptomyces</italic> sp. This strain showed an inhibiting rate of &gt;50% <italic>in vitro</italic> and biocontrol efficiency of 70.7% in sterile soil against <italic>Meloidogyne javanica</italic>, compared to the control. Greenhouse experiment results showed that the strain SA exhibits excellent biological control ability for plant-parasites both in roots and in root-knot nematode infested soil. SA treatment showed a higher number of bacterivores, especially <italic>Mesorhabditis</italic> and <italic>Cephalobus</italic>. The maturity index was significantly lower, while enrichment index (EI) was significantly higher in the SA treatment. In conclusion, this study presents an important potential application of the endophytic strain <italic>Streptomyces</italic> sp. for the control of plant-parasitic nematodes, especially <italic>Meloidogyne javanica</italic>, and presents the effects on the associated variation of the nematode community.</p>