AUTHOR=Jiao Jinzhen , Zhou Chuanshe , Guan L. L. , McSweeney C. S. , Tang Shaoxun , Wang Min , Tan Zhiliang 

TITLE=Shifts in Host Mucosal Innate Immune Function Are Associated with Ruminal Microbial Succession in Supplemental Feeding and Grazing Goats at Different Ages

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01655

DOI=10.3389/fmicb.2017.01655

ISSN=1664-302X

ABSTRACT=<p>Gastrointestinal microbiota may play an important role in regulating host mucosal innate immune function. This study was conducted to test the hypothesis that age (non-rumination, transition and rumination) and feeding type [Supplemental feeding (S) vs. Grazing (G)] could alter ruminal microbial diversity and maturation of host mucosal innate immune system in goat kids. MiSeq sequencing was applied to investigate ruminal microbial composition and diversity, and RT-PCR was used to test expression of immune-related genes in ruminal mucosa. Results showed that higher (<italic>P</italic> &lt; 0.05) relative abundances of <italic>Prevotella, Butyrivibrio, Pseudobutyrivibrio, Methanobrevibacter.gottschalkii, Neocallimastix, Anoplodinium–Diplodinium</italic>, and <italic>Polyplastron</italic>, and lower relative abundance of <italic>Methanosphaera</italic> (<italic>P</italic> = 0.042) were detected in the rumen of S kids when compared to those in G kids. The expression of genes encoding <italic>TLRs, IL1α, IL1β</italic> and <italic>TICAM2</italic> was down-regulated (<italic>P &lt;</italic> 0.01), while expression of genes encoding tight junction proteins was up-regulated (<italic>P &lt;</italic> 0.05) in the ruminal mucosa of S kids when compared to that in G kids. Moreover, irrespective of feeding type, relative abundances of ruminal <italic>Prevotella, Fibrobacter, Ruminococcus, Butyrivibrio, Methanobrevibacter, Neocallimastix</italic>, and <italic>Entodinium</italic> increased with age. The expression of most genes encoding <italic>TLRs</italic> and cytokines increased (<italic>P &lt;</italic> 0.05) from day 0 to 7, while expression of genes encoding tight junction proteins declined with age (<italic>P &lt;</italic> 0.05). This study revealed that the composition of each microbial domain changed as animals grew, and these changes might be associated with variations in host mucosal innate immune function. Moreover, supplementing goat kids with concentrate could modulate ruminal microbial composition, enhance barrier function and decrease local inflammation. The findings provide useful information in interpreting microbiota and host interactions, and developing nutritional strategies to improve the productivity and health of rumen during early life.</p>