AUTHOR=Yokoyama Masaru , Oka Tomoichiro , Takagi Hirotaka , Kojima Hirotatsu , Okabe Takayoshi , Nagano Tetsuo , Tohya Yukinobu , Sato Hironori TITLE=A Proposal for a Structural Model of the Feline Calicivirus Protease Bound to the Substrate Peptide under Physiological Conditions JOURNAL=Frontiers in Microbiology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01383 DOI=10.3389/fmicb.2017.01383 ISSN=1664-302X ABSTRACT=
Feline calicivirus (FCV) protease functions to cleave viral precursor proteins during productive infection. Previous studies have mapped a protease-coding region and six cleavage sites in viral precursor proteins. However, how the FCV protease interacts with its substrates remains unknown. To gain insights into the interactions, we constructed a molecular model of the FCV protease bound with the octapeptide containing a cleavage site of the capsid precursor protein by homology modeling and docking simulation. The complex model was used to screen for the substrate mimic from a chemical library by pharmacophore-based