AUTHOR=De Coninck Barbara , Verheesen Peter , Vos Christine M. , Van Daele Inge , De Bolle Miguel F. , Vieira Joao V. , Peferoen Marnix , Cammue Bruno P. A. , Thevissen Karin TITLE=Fungal Glucosylceramide-Specific Camelid Single Domain Antibodies Are Characterized by Broad Spectrum Antifungal Activity JOURNAL=Frontiers in Microbiology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01059 DOI=10.3389/fmicb.2017.01059 ISSN=1664-302X ABSTRACT=
Chemical crop protection is widely used to control plant diseases. However, the adverse effects of pesticide use on human health and environment, resistance development and the impact of regulatory requirements on the crop protection market urges the agrochemical industry to explore innovative and alternative approaches. In that context, we demonstrate here the potential of camelid single domain antibodies (VHHs) generated against fungal glucosylceramides (fGlcCer), important pathogenicity factors. To this end, llamas were immunized with purified fGlcCer and a mixture of mycelium and spores of the fungus