AUTHOR=Klafack Sandro , Wang Qing , Zeng Weiwei , Wang Yingying , Li Yingying , Zheng Shucheng , Kempter Jolanta , Lee Pei-Yu , Matras Marek , Bergmann Sven M. 

TITLE=Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00982

DOI=10.3389/fmicb.2017.00982

ISSN=1664-302X

ABSTRACT=<p>Worldwide koi herpesvirus (KHV) causes high mortalities in <italic>Cyprinus carpio</italic> L. aquaculture. So far, it is unknown how the different variants of KHV have developed and how they spread in the fish, but also in the environmental water bodies. Therefore, a phylogenetic method based on variable number of tandem repeats (VNTR) was improved to gain deeper insights into the phylogeny of KHV and its possible worldwide distribution. Moreover, a VNTR-3 qPCR was designed which allows fast virus typing. This study presents a useful method for molecular tracing of diverse KHV types, variants, and lineages.</p>