AUTHOR=Chen Yulin , Liu Shiliang A. , Mou Haijin , Ma Yunxiao , Li Meng , Hu Xiaoke 

TITLE=Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00871

DOI=10.3389/fmicb.2017.00871

ISSN=1664-302X

ABSTRACT=<p>Antibiotic resistance has become one of the world’s most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as <italic>Bacillus licheniformis by</italic> the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M+H]<sup>+</sup><italic>m/z</italic> 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was R<sub>n</sub>-Glu<sup>1</sup>-Leu/Ile<sup>2</sup>-Leu<sup>3</sup>-Val<sup>4</sup>-Asp<sup>5</sup>-Leu<sup>6</sup>-Leu/Ile<sup>7</sup>. The lipopeptides showed well resistance to UV light and the change of pH and temperature.</p>