AUTHOR=Aßmann Miriam , Mügge Carolin , Gaßmeyer Sarah Katharina , Enoki Junichi , Hilterhaus Lutz , Kourist Robert , Liese Andreas , Kara Selin 

TITLE=Improvement of the Process Stability of Arylmalonate Decarboxylase by Immobilization for Biocatalytic Profen Synthesis

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00448

DOI=10.3389/fmicb.2017.00448

ISSN=1664-302X

ABSTRACT=<p>The enzyme arylmalonate decarboxylase (AMDase) enables the selective synthesis of enantiopure (<italic>S</italic>)-arylpropinates in a simple single-step decarboxylation of dicarboxylic acid precursors. However, the poor enzyme stability with a half-life time of about 1.2 h under process conditions is a serious limitation of the productivity, which results in a need for high catalyst loads. By immobilization on an amino C2 acrylate carrier the operational stability of the (<italic>S</italic>)-selective AMDase variant G74C/M159L/C188G/V43I/A125P/V156L was increased to a half-life of about 8.6 days, which represents a 158-fold improvement. Further optimization was achieved by simple immobilization of the cell lysate to eliminate the cost- and time intensive enzyme purification step.</p>