AUTHOR=Li Yanan , Zheng Xiaoju , Zhang Xiujun , Bao Longfei , Zhu Yingying , Qu Yinbo , Zhao Jian , Qin Yuqi 

TITLE=The Different Roles of Penicillium oxalicum LaeA in the Production of Extracellular Cellulase and β-xylosidase

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.02091

DOI=10.3389/fmicb.2016.02091

ISSN=1664-302X

ABSTRACT=<p>Cellulolytic enzyme hydrolysis of lignocellulose biomass to release fermentable sugars is one of the key steps in biofuel refining. Gene expression of fungal cellulolytic enzymes is tightly controlled at the transcriptional level. Key transcription factors such as activator ClrB/CLR2 and XlnR/XYR1, as well as repressor CreA/CRE1 play crucial roles in this process. The putative protein methyltransferase LaeA/LAE1 has also been reported to regulate the gene expression of the cellulolytic enzyme. The formation and gene expression of the cellulolytic enzyme was compared among <italic>Penicillium oxalicum</italic> wild type (WT) and seven mutants, including Δ<italic>laeA</italic> (deletion of <italic>laeA</italic>), OE<italic>clrB</italic> (<italic>clrB</italic> overexpression), OE<italic>clrB</italic>Δ<italic>laeA</italic> (<italic>clrB</italic> overexpression with deletion of <italic>laeA</italic>), OE<italic>xlnR</italic> (<italic>xlnR</italic> overexpression), OE<italic>xlnR</italic>Δ<italic>laeA</italic> (<italic>xlnR</italic> overexpression with deletion of <italic>laeA</italic>), Δ<italic>creA</italic> (deletion of <italic>creA</italic>), and Δ<italic>creA</italic>Δ<italic>laeA</italic> (double deletion of <italic>creA</italic> and <italic>laeA</italic>). Results revealed that LaeA extensively affected the expression of glycoside hydrolase genes. The expression of genes that encoded the top 10 glycoside hydrolases assayed in secretome was remarkably downregulated especially in later phases of prolonged batch cultures by the deletion of <italic>laeA</italic>. Cellulase synthesis of four mutants Δ<italic>laeA</italic>, OE<italic>clrB</italic>Δ<italic>laeA</italic>, OE<italic>xlnR</italic>Δ<italic>laeA</italic>, and Δ<italic>creA</italic>Δ<italic>laeA</italic> was repressed remarkably compared with their parent strains WT, OE<italic>clrB</italic>, OE<italic>xlnR</italic>, and Δ<italic>creA</italic>, respectively. The overexpression of <italic>clrB</italic> or <italic>xlnR</italic> could not rescue the impairment of cellulolytic enzyme gene expression and cellulase synthesis when LaeA was absent, suggesting that LaeA was necessary for the expression of cellulolytic enzyme gene activated by ClrB or XlnR. In contrast to LaeA positive roles in regulating prominent cellulase and hemicellulase, the extracellular β-xylosidase formation was negatively regulated by LaeA. The extracellular β-xylosidase activities improved over 5-fold in the OE<italic>xlnR</italic>Δ<italic>laeA</italic> mutant compared with that of WT, and the expression of prominent β-xylosidase gene <italic>xyl3A</italic> was activated remarkably. The cumulative effect of LaeA and transcription factor XlnR has potential applications in the production of more β-xylosidase.</p>