AUTHOR=Jeanbille Mathilde , Gury Jérôme , Duran Robert , Tronczynski Jacek , Agogué Hélène , Ben Saïd Olfa , Ghiglione Jean-François , Auguet Jean-Christophe 

TITLE=Response of Core Microbial Consortia to Chronic Hydrocarbon Contaminations in Coastal Sediment Habitats

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.01637

DOI=10.3389/fmicb.2016.01637

ISSN=1664-302X

ABSTRACT=<p>Traditionally, microbial surveys investigating the effect of chronic anthropogenic pressure such as polyaromatic hydrocarbons (PAHs) contaminations consider just the alpha and beta diversity and ignore the interactions among the different taxa forming the microbial community. Here, we investigated the ecological relationships between the three domains of life (i.e., <italic>Bacteria, Archaea</italic>, and <italic>Eukarya</italic>) using 454 pyrosequencing on the 16S rRNA and 18S rRNA genes from chronically impacted and pristine sediments, along the coasts of the Mediterranean Sea (Gulf of Lion, Vermillion coast, Corsica, Bizerte lagoon and Lebanon) and the French Atlantic Ocean (Bay of Biscay and English Channel). Our approach provided a robust ecological framework for the partition of the taxa abundance distribution into 859 core Operational taxonomic units (OTUs) and 6629 satellite OTUs. OTUs forming the core microbial community showed the highest sensitivity to changes in environmental and contaminant variations, with salinity, latitude, temperature, particle size distribution, total organic carbon (TOC) and PAH concentrations as main drivers of community assembly. The core communities were dominated by <italic>Gammaproteobacteria</italic> and <italic>Deltaproteobacteria</italic> for <italic>Bacteria</italic>, by <italic>Thaumarchaeota, Bathyarchaeota</italic> and <italic>Thermoplasmata</italic> for <italic>Archaea</italic> and <italic>Metazoa</italic> and <italic>Dinoflagellata</italic> for <italic>Eukarya</italic>. In order to find associations among microorganisms, we generated a co-occurrence network in which PAHs were found to impact significantly the potential predator – prey relationship in one microbial consortium composed of ciliates and <italic>Actinobacteria</italic>. Comparison of network topological properties between contaminated and non-contaminated samples showed substantial differences in the network structure and indicated a higher vulnerability to environmental perturbations in the contaminated sediments.</p>