AUTHOR=Li Dan , Breiman Adrien , le Pendu Jacques , Uyttendaele Mieke 

TITLE=Anti-viral Effect of Bifidobacterium adolescentis against Noroviruses

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00864

DOI=10.3389/fmicb.2016.00864

ISSN=1664-302X

ABSTRACT=<p>This study aims to investigate the effect of <italic>Bifidobacterium adolescentis</italic> against noroviruses (NoVs). Murine norovirus-1 (MNV-1) used as a surrogate was detected by plaque assay and RT-qPCR. Human NoV virus like particles (VLPs) were detected by cell-binding assay. It was shown that the presence of <italic>B. adolescentis</italic> could inhibit the multiplication of MNV-1 on RAW 264.7 cells within 48 h of co-incubation period at 37°C. This inhibition did not occur at the viral binding stage, as no difference was observed in MNV-1 genomic copies collected from washed RAW 264.7 cells without and with <italic>B. adolescentis</italic> after co-incubation for 1 h at room temperature. Meanwhile, the presence of <italic>B. adolescentis</italic> decreased the binding of human NoV GI.1 VLPs to both Caco-2 cells and HT-29 cells, while no reduction was induced for the binding of human NoV GII.4 VLPs to Caco-2 cells.</p>