AUTHOR=Al Atya Ahmed K. , Belguesmia Yanath , Chataigne Gabrielle , Ravallec Rozenn , Vachée Anne , Szunerits Sabine , Boukherroub Rabah , Drider Djamel 

TITLE=Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00817

DOI=10.3389/fmicb.2016.00817

ISSN=1664-302X

ABSTRACT=<p>Methicillin-resistant <italic>Staphylococcus aureus</italic> (MRSA) has become a worrisome superbug. This work aimed at studying the effects of two class IIb bacteriocins, enterocins DD28 and DD93 as anti-MRSA agents. Thus, these bacteriocins were purified, from the cultures supernatants of <italic>Enterococcus faecalis</italic> 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. The anti-Staphylococcal activity was shown <italic>in vitro</italic> by the assessment of the minimal inhibitory concentration (MIC), followed by a checkerboard and time-kill kinetics experiments. The data unveiled a clear synergistic effect of enterocins DD28 and DD93 in combination with erythromycin or kanamycin against the clinical MRSA-S1 strain. Besides, these combinations impeded as well the MRSA-S1 clinical strain to setup biofilms on stainless steel and glace devices.</p>