AUTHOR=Joshi Sumit , Yadav Narendra K. , Rawat Keerti , Tripathi Chandra Dev P. , Jaiswal Anil K. , Khare Prashant , Tandon Rati , Baharia Rajendra K. , Das Sanchita , Gupta Reema , Kushawaha Pramod K. , Sundar Shyam , Sahasrabuddhe Amogh A. , Dube Anuradha 

TITLE=Comparative Analysis of Cellular Immune Responses in Treated Leishmania Patients and Hamsters against Recombinant Th1 Stimulatory Proteins of Leishmania donovani

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00312

DOI=10.3389/fmicb.2016.00312

ISSN=1664-302X

ABSTRACT=<p>Our prior studies demonstrated that cellular response of T helper 1 (Th1) type was generated by a soluble antigenic fraction (ranging from 89.9 to 97.1 kDa) of <italic>Leishmania donovani</italic> promastigote, in treated <italic>Leishmania</italic> patients as well as hamsters and showed significant prophylactic potential against experimental visceral leishmaniasis (VL). Eighteen Th1 stimulatory proteins were identified through proteomic analysis of this subfraction, out of which 15 were developed as recombinant proteins. In the present work, we have evaluated these 15 recombinant proteins simultaneously for their comparative cellular responses in treated <italic>Leishmania</italic> patients and hamsters. Six proteins <italic>viz.</italic> elongation factor-2, enolase, aldolase, triose phosphate isomerase, protein disulfide isomerase, and p45 emerged as most immunogenic as they produced a significant lymphoproliferative response, nitric oxide generation and Th1 cytokine response in PBMCs and lymphocytes of treated <italic>Leishmania</italic> patients and hamsters respectively. The results suggested that these proteins may be exploited for developing a successful poly-protein and/or poly-epitope vaccine against VL.</p>