AUTHOR=Dai Xiaotian , Zhou Dongsheng , Xiong Wei , Feng Jiao , Luo Wenbo , Luo Guangming , Wang Haijing , Sun Fengjun , Zhou Xiangdong 

TITLE=The IncP-6 Plasmid p10265-KPC from Pseudomonas aeruginosa Carries a Novel ΔISEc33-Associated blaKPC-2 Gene Cluster

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00310

DOI=10.3389/fmicb.2016.00310

ISSN=1664-302X

ABSTRACT=<p><italic>Pseudomonas aeruginosa</italic> strain 10265 was recovered from a patient with pneumonia in a Chinese public hospital, and it displays the carbapenem resistance phenotype due to the acquisition of a non-conjugative but mobilizable IncP-6-type plasmid p10265-KPC. p10265-KPC carries a Tn<italic>5563</italic>-borne defective <italic>mer</italic> locus, and a novel ΔIS<italic>Ec33</italic>-associated <italic>bla</italic><sub>KPC-2</sub> gene cluster without paired inverted repeats and paired direct repeats at both ends. Mobilization of this ΔIS<italic>Ec33</italic>-associated element in p10265-KPC would be attributed to homologous recombination-based insertion of a foreign structure Tn<italic>3-</italic>IS<italic>Apu1</italic>-<italic>orf7</italic>-IS<italic>Apu2</italic>- IS<italic>Kpn27-</italic>Δ<italic>bla</italic><sub>TEM-1</sub><italic>-bla</italic><sub>KPC-2</sub><italic>-</italic>ΔIS<italic>Kpn6- korC-orf6</italic>-<italic>klcA-</italic>Δ<italic>repB</italic> into a pre-existent intact IS<italic>Ec33</italic>, making IS<italic>Ec33</italic> truncated at the 3′ end. The previously reported pCOL-1 represents the first sequenced KPC-producing IncP-6 plasmid, while p10265-KPC is the second one. These two plasmids carry two distinct <italic>bla</italic><sub>KPC-2</sub> gene clusters, which are inserted into the different sites of the IncP-6 backbone and have different evolutionary histories of assembly and mobilization. This is the first report of identification of the IncP-6-type resistance plasmid in China.</p>