AUTHOR=Gu Haiping , Lou Jun , Wang Haizhen , Yang Yu , Wu Laosheng , Wu Jianjun , Xu Jianming 

TITLE=Biodegradation, Biosorption of Phenanthrene and Its Trans-Membrane Transport by Massilia sp. WF1 and Phanerochaete chrysosporium

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00038

DOI=10.3389/fmicb.2016.00038

ISSN=1664-302X

ABSTRACT=<p>Reducing phenanthrene (PHE) in the environment is critical to ecosystem and human health. Biodegradation, biosorption, and the trans-membrane transport mechanism of PHE by a novel strain, <italic>Massilia</italic> sp. WF1, and an extensively researched model fungus, <italic>Phanerochaete chrysosporium</italic> were investigated in aqueous solutions. Results showed that the PHE residual concentration decreased with incubation time and the data fitted well to a first-order kinetic equation, and the <italic>t</italic><sub>1/2</sub> of PHE degradation by WF1, spores, and mycelial pellets of <italic>P</italic>. <italic>chrysosporium</italic> were about 2 h, 87 days, and 87 days, respectively. The biosorbed PHE was higher in <italic>P</italic>. <italic>Chrysosporium</italic> than that in WF1, and it increased after microorganisms were inactivated and inhibited, especially in mycelial pellets. The detected intracellular auto-fluorescence of PHE by two-photon excitation microscopy also proved that PHE indeed entered into the cells. Based on regression, the intracellular (<italic>K</italic><sub>din</sub>) and extracellular (<italic>K</italic><sub>dout</sub>) dissipation rate constants of PHE by WF1 were higher than those by spores and mycelial pellets. In addition, the transport rate constant of PHE from outside solution into cells (<italic>K<sub>in</sub>S/V<sub>out</sub></italic>) for WF1 were higher than the efflux rate constant of PHE from cells to outside solution (<italic>K<sub>out</sub>S/V<sub>in</sub></italic>), while the opposite phenomena were observed for spores and mycelial pellets. The amount of PHE that transported from outside solution into cells was attributed to the rapid degradation and active PHE efflux in the cells of WF1 and <italic>P</italic>. <italic>Chrysosporium</italic>, respectively. Besides, the results under the inhibition treatments of 4°C, and the presence of sodium azide, colchicine, and cytochalasin B demonstrated that a passive trans-membrane transport mechanism was involved in PHE entering into the cells of WF1 and <italic>P</italic>. <italic>Chrysosporium</italic>.</p>