AUTHOR=Qi Xin , Zha Jian , Liu Gao-Gang , Zhang Weiwen , Li Bing-Zhi , Yuan Ying-Jin 

TITLE=Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.01165

DOI=10.3389/fmicb.2015.01165

ISSN=1664-302X

ABSTRACT=<p>Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in <italic>Saccharomyces cerevisiae</italic>. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of <italic>XylA</italic> and endogenous <italic>XKS1</italic>. Additional overexpression of genes <italic>RKI1, RPE1, TKL1</italic>, and <italic>TAL1</italic> in the non-oxidative pentose phosphate pathway (PPP) in SyBE001 increased the xylose consumption rate by 1.19-fold. By repetitive adaptation, the xylose utilization rate was further increased by ∼10-fold in the resultant strain SyBE003. Gene expression analysis identified a variety of genes with significantly changed expression in the PPP, glycolysis and the tricarboxylic acid cycle in SyBE003.</p>