AUTHOR=Bei Weiya , Zhou Yan , Xing Xuya , Zahi Mohamed Red , Li Yuan , Yuan Qipeng , Liang Hao 

TITLE=Organogel-nanoemulsion containing nisin and D-limonene and its antimicrobial activity

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.01010

DOI=10.3389/fmicb.2015.01010

ISSN=1664-302X

ABSTRACT=<p>The aim of this study was to investigate a novel delivery system containing <sc>D</sc>-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity. Organogel-nanoemulsion containing with <sc>D</sc>-limonene and nisin or without nisin was prepared by a homogenization method. Factors that may affect the droplet size and stability of organogel-nanoemulsion such as pressure and surfactant to oil ratio (SOR) were studied. The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low SOR. Positive effects and outstanding antimicrobial activities of organogel-nanoemulsion containing with <sc>D</sc>-limonene and nisin were confirmed by minimal inhibitory concentrations comparison, growth curves of bacteria, scanning electron microscopy and determination of cell constituents’ release. Furthermore, the organogel-nanoemulsion applied as food preservative in milk also shown excellent antimicrobial performance. Overall, the research described in the current article show that organogel-nanoemulsion containing with <sc>D</sc>-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.</p>