AUTHOR=Ser Hooi-Leng , Palanisamy Uma D. , Yin Wai-Fong , Abd Malek Sri N. , Chan Kok-Gan , Goh Bey-Hing , Lee Learn-Han 

TITLE=Presence of antioxidative agent, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- in newly isolated Streptomyces mangrovisoli sp. nov.

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00854

DOI=10.3389/fmicb.2015.00854

ISSN=1664-302X

ABSTRACT=<p>A novel <italic>Streptomyces</italic>, strain MUSC 149<sup>T</sup> was isolated from mangrove soil. A polyphasic approach was used to study the taxonomy of MUSC 149<sup>T</sup>, which shows a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus <italic>Streptomyces</italic>. The diamino acid of the cell wall peptidoglycan was LL-diaminopimelic acid. The predominant menaquinones were identified as MK9(H<sub>8</sub>) and MK9(H<sub>6</sub>). Phylogenetic analysis indicated that closely related strains include <italic>Streptomyces rhizophilus</italic> NBRC 108885<sup>T</sup> (99.2% sequence similarity), <italic>S. gramineus</italic> NBRC 107863<sup>T</sup> (98.7%) and <italic>S. graminisoli</italic> NBRC 108883<sup>T</sup> (98.5%). The DNA–DNA relatedness values between MUSC 149<sup>T</sup> and closely related type strains ranged from 12.4 ± 3.3% to 27.3 ± 1.9%. The DNA G + C content was determined to be 72.7 mol%. The extract of MUSC 149<sup>T</sup> exhibited strong antioxidant activity and chemical analysis reported identification of an antioxidant agent, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-. These data showed that metabolites of MUSC 149<sup>T</sup> shall be useful as preventive agent against free-radical associated diseases. Based on the polyphasic study of MUSC 149<sup>T</sup>, the strain merits assignment to a novel species, for which the name <italic>S. mangrovisoli</italic> sp. nov. is proposed. The type strain is MUSC 149<sup>T</sup> (=MCCC 1K00699<sup>T</sup>=DSM 100438<sup>T</sup>).</p>