AUTHOR=Dziewit Lukasz , Czarnecki Jakub , Prochwicz Emilia , Wibberg Daniel , Schlüter Andreas , Pühler Alfred , Bartosik Dariusz 

TITLE=Genome-guided insight into the methylotrophy of Paracoccus aminophilus JCM 7686

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00852

DOI=10.3389/fmicb.2015.00852

ISSN=1664-302X

ABSTRACT=<p><italic>Paracoccus aminophilus</italic> JCM 7686 (<italic>Alphaproteobacteria</italic>) is a facultative, heterotrophic methylotroph capable of utilizing a wide range of C1 compounds as sole carbon and energy sources. Analysis of the JCM 7686 genome revealed the presence of genes involved in the oxidation of methanol, methylamine, dimethylamine, trimethylamine, <italic>N</italic>,<italic>N</italic>-dimethylformamide, and formamide, as well as the serine cycle, which appears to be the only C1 assimilatory pathway in this strain. Many of these genes are located in different extrachromosomal replicons and are not present in the genomes of most members of the genus <italic>Paracoccus</italic>, which strongly suggests that they have been horizontally acquired. When compared with <italic>Paracoccus denitrificans</italic> Pd1222 (type strain of the genus <italic>Paracoccus</italic>), <italic>P. aminophilus</italic> JCM 7686 has many additional methylotrophic capabilities (oxidation of dimethylamine, trimethylamine, <italic>N,N</italic>-dimethylformamide, the serine cycle), which are determined by the presence of three separate gene clusters. Interestingly, related clusters form compact methylotrophy islands within the genomes of <italic>Paracoccus</italic> sp. N5 and many marine bacteria of the <italic>Roseobacter</italic> clade.</p>