AUTHOR=Cao Changfu , Qu Ying , Sun Meizhen , Qiu Zhenzhen , Huang Xianhui , Huai Binbin , Lu Yan , Zeng Zhenling 

TITLE=In vivo antimicrobial activity of marbofloxacin against Pasteurella multocida in a tissue cage model in calves

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00759

DOI=10.3389/fmicb.2015.00759

ISSN=1664-302X

ABSTRACT=<p>Marbofloxacin is a fluoroquinolone specially developed for use in veterinary medicine with broad-spectrum antibacterial activity. The objective of our study was to re-evaluate <italic>in vivo</italic> antimicrobial activity of marbofloxacin against <italic>Pasteurella multocida</italic> using subcutaneously implanted tissue cages in calves. Calves were infected by direct injection into tissue cages with <italic>P. multocida</italic>(type B, serotype 2), then intramuscularly received a range of marbofloxacin doses 24 h after inoculation. The ratio of 24 h area under the concentration-time curve divided by the minimum inhibitory concentration or the mutant prevention concentration (AUC<sub>24</sub><sub>h</sub>/MIC or AUC<sub>24</sub><sub>h</sub>/MPC) was the pharmacokinetic-pharmacodynamic (PK/PD) index that best described the effectiveness of marbofloxacin against <italic>P. multocida</italic> (<italic>R</italic><sup>2</sup> = 0.8514) by non-linear regression analysis. Marbofloxacin exhibited a good antimicrobial activity <italic>in vivo</italic>. The levels of AUC<sub>24</sub><sub>h</sub>/MIC and AUC<sub>24</sub><sub>h</sub>/MPC that produced 50% (1.5log<sub>10</sub> CFU/mL reduction) and 90% (3log<sub>10</sub> CFU/mL reduction) of maximum response were 18.60 and 50.65 h, 4.67 and 12.89 h by using sigmoid <italic>E<sub>max</sub></italic> model WINNONLIN software, respectively. The <italic>in vivo</italic> PK/PD integrated methods by tissue cage model display the advantage of the evaluation of antimicrobial activity and the optimization of the dosage regimen for antibiotics in the presence of the host defenses, especially in target animal of veterinary interest.</p>