AUTHOR=Pontes Bruno , Frases Susana 

TITLE=The Cryptococcus neoformans capsule: lessons from the use of optical tweezers and other biophysical tools

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00640

DOI=10.3389/fmicb.2015.00640

ISSN=1664-302X

ABSTRACT=<p>The fungal pathogen <italic>Cryptococcus neoformans</italic> causes life-threatening infections in immunocompromised individuals, representing one of the leading causes of morbidity and mortality in AIDS patients. The main virulence factor of <italic>C. neoformans</italic> is the polysaccharide capsule; however, many fundamental aspects of capsule structure and function remain poorly understood. Recently, important capsule properties were uncovered using optical tweezers and other biophysical techniques, including dynamic and static light scattering, zeta potential and viscosity analysis. This review provides an overview of the latest findings in this emerging field, explaining the impact of these findings on our understanding of <italic>C. neoformans</italic> biology and resistance to host immune defenses.</p>