AUTHOR=Zhang Huajun , Zhang Su , Peng Yun , Li Yi , Chen Zhangran , Xu Hong , Yu Zhiming , Zheng Wei , Zheng Tianling 

TITLE=Effects of marine actinomycete on the removal of a toxicity alga Phaeocystis globose in eutrophication waters

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00474

DOI=10.3389/fmicb.2015.00474

ISSN=1664-302X

ABSTRACT=<p><italic>Phaeocystis globosa</italic> blooms in eutrophication waters can cause severely damage in marine ecosystem and consequently influence human activities. This study investigated the effect and role of an algicidal actinomycete (<italic>Streptomyces</italic> sp. JS01) on the elimination process of <italic>P. globosa</italic>. JS01 supernatant could alter algal cell membrane permeability in 4 h when analyzed with flow cytometry. Reactive oxygen species (ROS) levels were 7.2 times higher than that at 0 h following exposure to JS01 supernatant for 8 h, which indicated that algal cells suffered from oxidative damage. The Fv/Fm value which could reflect photosystem II (PS II) electron flow status also decreased. Real-time PCR showed that the expression of the photosynthesis related genes <italic>psbA</italic> and <italic>rbcS</italic> were suppressed by JS01 supernatant, which might induce damage to PS II. Our results demonstrated that JS01 supernatant can change algal membrane permeability in a short time and then affect photosynthesis process, which might block the PS II electron transport chain to produce excessive ROS. This experiment demonstrated that <italic>Streptomyces</italic> sp. JS01 could eliminate harmful algae in marine waters efficiently and may be function as a harmful algal bloom controller material.</p>