AUTHOR=Farci Domenica , Bowler Matthew W. , Esposito Francesca , McSweeney Sean , Tramontano Enzo , Piano Dario 

TITLE=Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00414

DOI=10.3389/fmicb.2015.00414

ISSN=1664-302X

ABSTRACT=<p>The protein DR_2577 is a major Surface layer component of the radio-resistant bacterium <italic>Deinococcus radiodurans</italic>. In the present study DR_2577 has been purified and its oligomeric profile characterized by means of size exclusion chromatography and gel electrophoresis. DR_2577 was found to be organized into three hierarchical orders characterized by monomers, stable dimers formed by the occurrence of disulfide bonds, and hexamers resulting from a combination of dimers. The structural implications of these findings are discussed providing new elements for a more integrated model of this S-layer.</p>